RESUMEN
Background: In most serious COVID-19 forms which required prolonged stay in intensive care unit, pulmonary, cardiovascular, renal, neurological and psychological sequelae have been reported after the infection. All these complications can be sustained by chronic inflammatory problems and/ or increased oxidative stress. Material and Methods: Biomarkers of the systemic oxidative stress status (OSS) including enzymatic and non-enzymatic antioxidants, total antioxidant capacity of plasma (PAOT®-Sore), trace elements, oxidative damage to lipids and inflammation markers, were investigated in 12 patients admitted to a revalidation center for post-19 COVID pneumonia. Results: From blood samples collected two months after hospital discharge and one month after admission to the revalidation center, vitamin C, thiol proteins, reduced glutathione, gamma-tocopherol and beta carotene were significantly decreased compared to reference values. By contrast, lipid peroxides and markers of inflammation (neutrophils, myeloperoxidase) were significantly higher than the norms. Lipid peroxides was strongly correlated with Cu (r = 0.95, P < 0.005) and Cu/Zn ratio (0.66, P = 0.020). Using an electrochemical method (PAOT®), total antioxidant capacity (TAC) evaluated in saliva and urine negatively correlated with copper and lipid peroxides. Similar findings were obtained for PAOT®-skin score. Conclusions: Systemic OSS was strongly altered in patients admitted in revalidation after C0VID-19 infection. This suggests the need for supplementing these patients with antioxidants.
RESUMEN
Background: A key role of oxidative stress has been highlighted in the pathogenesis of COVID-19. However, little has been said about oxidative stress status (OSS) of COVID-19 patients hospitalized in intensive care unit (ICU). Material and Methods: Biomarkers of the systemic OSS included antioxidants (9 assays), trace elements (3 assays), inflammation markers (4 assays) and oxidative damage to lipids (3 assays). Results: Blood samples were drawn after 9 (7–11) and 41 (39–43) days of ICU stay, respectively in 3 and 6 patients. Vitamin C, thiol proteins, reduced glutathione, γ-tocopherol, β-carotene and PAOT® score were significantly decreased compared to laboratory reference values. Selenium concentration was at the limit of the lower reference value. By contrast, the copper/zinc ratio (as a source of oxidative stress) was higher than reference values in 55% of patients while copper was significantly correlated with lipid peroxides (r = 0.95, p < 0.001). Inflammatory biomarkers (C-reactive protein and myeloperoxidase) were significantly increased when compared to normals. Conclusions: The systemic OSS was strongly altered in critically ill COVID-19 patients as evidenced by increased lipid peroxidation but also by deficits in some antioxidants (vitamin C, glutathione, thiol proteins) and trace elements (selenium).
RESUMEN
Post COVID-19 sequelae include several complications including cognitive impairment, a situation associated with increased oxidative stress. In a pilot study, we intend to recruit 20 patients having a MOCA (MOntreal Cognitive Assessment questionnaire) score ≤ 25 after their discharge from hospital for COVID-19 infection requiring a long stay (> 30 days) in Intensive Care Unit. The goal of our study was to check how a blend of polyphenols (French Grape (Vitis vinifera L.) and North-American Wild Blueberry (Vaccinium angustifolium A) extracts)) at 800 mg enriched with vitamins and minerals at nutritional doses (Memophenol™l) and given each day during 6 months could potentially decrease oxidative stress and improve cognitive status when compared to a placebo. For that, we propose to investigate a large battery of tests including the determination of antioxidants (vitamins C and E (alpha- and gamma-tocopherol), beta-carotene, glutathione, thiol proteins, total polyphenols, paraoxonase, glutathione peroxidase), trace elements (copper, zinc, selenium), oxidative damages to lipids (lipid peroxides, oxidized LDL) and inflammatory biomarkers (myeloperoxidase), respectively before supplementation, 3 and 6 months after. In parallel, the evolution of the MOCA score will be followed. Actually 8 patients have been included in the study.